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fix(cladistics): merge #223 with main and preserve unplaced placement mass - #230
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… and repair the CladeCumulus readers `main` still cannot load the package, and the CladeCumulus suite that never ran before now reports twelve defects. All of them are narrowed to: Package loading - src/core/doi.jl: #210 removed `module DOI` but left `end # module DOI`, a lowering error at include time ("end" at top level) — every test file fails before it starts. The opener is restored. - src/server/server.jl: routes/cladistics.jl was included twice, so every /cladistics route is registered twice. One include remains. CladeCumulus readers (test_clade_cumulus.jl, 3 failed / 14 errored) - routes/clade_cumulus.jl opened results.duckdb with `DuckDB.DB` and `DBInterface.connect` without importing either module, so the sources route answered 500 and every HTTP assertion downstream of it failed. Both are imported now, as the other route files already do. - `_graft_jplace_queries` and the diversity block in the route still used the pre-#207 field names (`weight`, `distal_length`, `pendant_length`) on `QueryCandidate` (whose fields are `like_weight`, `distal_len`, `pendant_len`), and `_graft_jplace_queries` used `.edge_num` on `PhyloNode` (whose field is `edge_number`). Five FieldErrors, now aligned with the structs. - parse_newick lost NHX support: the label reader consumed `[&&NHX:B=92]` before the call that records it, so `) [&&NHX:B=92] :0.1` parsed with support `nothing`. The reader now takes the capture refs. - parse_jplace rejected a single-candidate placement whose ratio is below 1.0, which is how placement tools report one site: the remainder is mass they did not place. A single candidate is now decisive when its ratio is at least the new `PLACEMENT_SINGLE_MIN_LWR = 0.5`; a multi-candidate placement must still partition the mass to within `PLACEMENT_WEIGHT_SUM_TOL`. A single candidate below half stays `undecided` with the same reason string. - test_clade_cumulus.jl reached the tool registry as `PH.Tools`, but `Phylogeny` imports `tool_bin` selectively and does not bind `Tools`; `MetaManifold.Tools` is the module that owns the registry. Guards against both merge-harm classes - scripts/check-julia-syntax.jl now rejects a bare `end` at the top level. Such a file parses cleanly and only fails at lowering, which is exactly how a missing `module` opener — the failure that stopped the whole suite — passed the parse gate. - test_routes.jl asserts that no method+path pair is registered twice and that both cladistics route files are present, which no request test could reveal (a duplicate registration silently shadows the first). Co-authored-by: arena-agent <297053741+arena-agent@users.noreply.github.com>
`main` landed #222 while #223 was open, and the two repaired an overlapping set of CladeCumulus defects by different routes. This merge keeps every fix exactly once and settles the one disagreement between them. Textual conflicts (both sides fixed the same defect): * `read_label_and_quoted` NHX capture — kept main's keyword signature, which the call sites already use, with #223's clearer comment. * `PH.Tools` -> `MetaManifold.Tools` in the FastTree testset — identical on both sides; kept #223's note explaining why. Semantic conflict (git merged both halves into an incoherent rule): * #222 widened `PLACEMENT_WEIGHT_SUM_TOL` from 1e-5 to 0.05 so a lone 0.98 placement would be accepted; #223 kept 1e-5 and added an explicit single-candidate rule. Merging both left the 0.05 slack applied only to multi-candidate partitions, which is where it is least wanted. Resolved to #223's model: a multi-candidate record partitions the mass and must still sum to one within 1e-5, while a single-candidate record is judged on its own share against `PLACEMENT_SINGLE_MIN_LWR`. A truncated 0.96 partition is caught again, and a lone 0.98 still passes. Codex review finding (P1, `routes/clade_cumulus.jl`) — preserve unplaced LWR mass in diversity weights: * Accepting a single candidate down to LWR 0.5 made the diversity block's `cand.like_weight / tw` always 1.0, pushing mass the tool had explicitly left unplaced onto the reported edge and inflating that tip in weighted UniFrac and Faith's PD. Tips are now weighted by the raw like-weight ratio, and the shortfall is reported as a new `unplaced_reads` field per sample rather than renormalised away. * `_graft_jplace_queries` is deliberately unchanged: the clade tree is a count view of the observed table and still conserves every read. The difference between the two consumers is documented at the constants. Also from #223, unchanged: the `module DOI` opener, the duplicate `routes/cladistics.jl` include, the `edge_number`/`like_weight` field names, the stray-top-level-`end` gate in `scripts/check-julia-syntax.jl`, and the duplicate-route-registration test. Tests: the two-rule acceptance model is pinned at the parser, and a new route case drives a `.jplace` study tree through `/clade-cumulus` and asserts both the reported unplaced reads and that the clade tree still conserves all 110 reads.
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CI on `main` (8df1041) shows `POST /clade-cumulus` answering 500 for every `study_tree` request: MethodError: no method matching _valid_tree_file(::SubString{String}) `JSON3` hands request fields back as `SubString`s and `strip` keeps them that way, but `_valid_tree_file` and `_tree_format` in `routes/trees.jl` are `String`-typed. The handler has no catch around `_resolve_tree_inputs_from_request`, so the MethodError surfaced as a 500 and took seven assertions down with it: `r_comb.status == 200` failed, and every `comb.*` access after it raised `KeyError` until the testset aborted. #222 fixed this class at `_load_run_table_df` -- "JSON3 hands request fields back as SubStrings; the DuckDB helpers are String-typed, so normalise once at this boundary" -- but `_resolve_tree_inputs_from_request` two functions below kept the unnormalised values. `cladistics.jl` already wraps its own call as `_valid_tree_file(String(published))`, so the convention is to normalise at the call site; this follows it. The existing `r_comb` assertion is the regression test: it sends `"treeFile" => "amplicon.nwk"` over JSON, which is exactly the SubString path that broke. With the `u.edge_num` -> `u.edge_number` repair already carried in from #223, this accounts for 8 of the 9 errors that run reported.
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| row_weight = Dict{Int,Float64}() | ||
| for (ri, w) in taxa_rows | ||
| row_weight[ri] = get(row_weight, ri, 0.0) + w |
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Count fully unmapped ASVs as unplaced
When an ASV has an undecided placement or is absent from the jplace placements, it is not grafted into phylo_root and therefore never appears in taxa_rows. Building row_weight only from taxa_rows omits that ASV entirely instead of assigning it a placed weight of zero, so unplaced_reads excludes 100% of its per-sample reads and can misleadingly report zero loss. Include table rows with no mapped tip when calculating the shortfall.
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| # `parse_jplace` has already rejected any ratio outside | ||
| # [0, 1], so this needs no further clamping. | ||
| tip_weights[tid] = cand.like_weight |
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Preserve unplaced mass through UniFrac normalization
When a sample has a partially placed query—especially if it is the sample's only mapped query—storing the raw LWR here does not keep the missing mass out of weighted UniFrac: weighted_unifrac later computes its denominator as sum(mat[:, sample]) and divides every branch abundance by that placed-only total. A lone 0.6 placement therefore becomes relative abundance 1.0 again and produces exactly the certainty inflation this change is intended to remove; unplaced_reads is only reported as metadata and does not affect the matrices. The UniFrac calculation needs the original per-sample total (or an explicit off-tree component) as its normalization denominator.
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… placed mass Codex raised two findings on the diversity block, and they are the same defect seen from two sides. Retaining the raw like-weight ratio stopped a lone 0.6 placement from being inflated to 1.0 in `mat`, but `weighted_unifrac` then divided by `sum(values(ab))` -- the placed-only mass -- which restored exactly the weight that had just been withheld. The same denominator also erased ASVs the tree does not cover: a sample could be 90% unmapped and still be compared as though every read had been placed. `_sample_total` now lets a caller declare the real library size, and `weighted_unifrac`, `branch_unifrac_contributions` and `unifrac_matrix` take it (`total_a`/`total_b`/`totals`). All default to `nothing` and the mass on the tree, so every existing call is unchanged. A declared total below the on-tree mass is rejected. The route computes one denominator per sample -- the sum of the table column -- and reports `library size - on-tree mass` as `unplaced_reads`, which now covers the like-weight shortfall and uncovered ASVs together. Faith's PD and unweighted UniFrac read presence only and are untouched. Two unrelated defects the suite could not previously reach: `_reaggregate_after_move` built `subtree_pd` by summing each child's branch length plus its subtree, which drops the branch subtending the node itself -- the opposite of `clade_faith_pd`, which the builder uses. Every proposed clade therefore came back short by its own branch and was compared against a current tree measured the other way: moving ASV_4 into a clade of PD 0.60 reported 0.55. It now mirrors `_pd_accum`, and the test pins the exact values plus total-PD conservation. `test_clade_cumulus.jl` bound `const SV`; the thirteen later unit files that bind `SV = MetaManifold.Server` plainly then threw `invalid assignment to constant Main.SV`. The throw escaped the include loop in `runtests.jl`, so roughly thirty unit files and the occupancy probe never ran at all. The binding is now plain, and each file is included inside its own testset so a top-level throw is attributed to its file and does not take the rest of the suite down with it.
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Review comments at @frontend/src/components/CladeCumulusPanel.tsx:
- Around line 723-725: Correct the unplaced-reads description in
CladeCumulusPanel and the matching description in the API types so it states
that unplaced reads are included in each sample’s library-size denominator but
excluded from tree-tip abundances.
Review comments at @src/analysis/clade_cumulus.jl:
- Line 1878: Update the activity and PD calculation near `leaf_pd` to recompute
an emptied clade’s count from `direct_count` before determining `active_here`,
so a clade left empty by `graft` does not retain activity from its stale
`cumulative_count`.
Review comments at @test/unit/test_routes.jl:
- Around line 956-957: Update the route assertions in the test to check
membership of the exact registered paths for `/cladistics` and
`/clade-cumulus/sources`, rather than using `occursin`, so longer or incorrectly
named routes cannot satisfy them.
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| Unplaced reads (carried by no tip — like-weight mass left on no edge, plus | ||
| ASVs the tree does not cover — and held out of the weighted UniFrac | ||
| denominators):{' '} |
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🎯 Functional Correctness | 🟡 Minor | ⚡ Quick win
Correct the weighted UniFrac denominator description.
The route includes unplaced reads in each sample’s library-size denominator. It excludes those reads from tree-tip abundances. This text says the reads are held out of the denominators, which reverses the reported calculation. Correct this text and the matching description in frontend/src/api/types.ts, Lines 975–976.
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| # which the builder uses, counts a node's OWN branch when the | ||
| # subtree below it holds an observed tip -- so an observed tip | ||
| # contributes its own branch length, not zero. | ||
| leaf_pd = (active_here && !is_root) ? max(0.0, u.branch_length) : 0.0 |
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🎯 Functional Correctness | 🟡 Minor | ⚡ Quick win
Recompute activity when a move empties a clade.
If a clade has one child and that child moves elsewhere, graft leaves the clade empty but retains its old cumulative_count. active_here then remains true, so this line assigns the empty clade a non-zero subtree_pd. Recompute the emptied clade’s count from direct_count before calculating its activity and PD.
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With the include loop no longer aborting, `test_differential.jl` and the server smoke tests ran for the first time and found three defects. `_wald` called `erfc`, which exists in no module this project loads -- `SpecialFunctions` is deliberately not a dependency, and the header of `compositional.jl` says so. Every multinomial and Dirichlet-multinomial fit therefore died with `UndefVarError: erfc` the moment it tried to report a p-value, which is to say the models could not be used at all. `compositional_erfc` now sits beside `compositional_loggamma` and `compositional_digamma` and is built the same way, from the regularised incomplete gamma function at a = 1/2: the power series for P below x^2 = 1.5 and the Legendre continued fraction for Q above it, needing only `exp` and `log`. Measured against the C library's erfc the relative error stays under 1e-14 wherever erfc(x) >= 1e-50, i.e. out to a two-sided deviate of |z| = 15, which matches the accuracy the other two already promise. The unit tests pin both sides of the crossover, the reflection, and the two-sided p-value that is its only caller. `min_prevalence` is the second positional argument of `DifferentialConfig`, not a keyword, so the "every taxon filtered out" case constructed a `MethodError` instead of reaching the engine. The same call also passed four samples that were all in group A, which the "each group needs at least one sample" guard would have answered long before the prevalence filter. Both are fixed, so the case now asserts what it says it does.
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Main landed #226 while this branch was in review, and the two found three of the same defects independently. Main's resolution is taken for all three, since it is already on main: - `erfc`: main's private `_erfc` replaces the `compositional_erfc` added here. Same algorithm (incomplete gamma at a = 1/2, series below x^2 = 1.5 and a Lentz continued fraction above), so only the name and the tests differ; `compositional.jl` and `test_differential.jl` are taken wholesale from main. - `subtree_pd` in `_reaggregate_after_move`: identical fix, main's `own_pd` naming and one-line post-loop form kept. Git had merged the two variants additively, which would have counted every active internal branch twice; that is resolved to a single addition. - The `Main.SV` collision that was aborting the include loop: main renames this file's binding to `CLADE_SV` instead of dropping `const`. Main's rename is kept. - `min_prevalence` in the multinomial engine test: main's eight-sample table is better than the four-sample one here, and is kept. Kept from this branch, because main does not have them: - The raw like-weight ratio in the diversity block. Main's change to that line only renames `c.weight` to `c.like_weight` and keeps `cand.like_weight / tw`, which is the renormalisation Codex objected to on #223: it hands a lone 0.6 placement a weight of 1.0. The divisor is removed, and the mass the tool never placed is reported as `unplaced_reads`. - `_sample_total` and the `total_a`/`total_b`/`totals` arguments on `weighted_unifrac`, `branch_unifrac_contributions` and `unifrac_matrix`, with the route passing each sample's library size. Without them the metric divides by the placed-only mass and undoes the line above. - Per-file testsets in `runtests.jl`. Main fixed the `SV` collision but not the reason one bad binding could silently skip thirty-five unit files and the occupancy probe. - `_cc_find`, and the drag-and-drop assertions that pin the exact post-move PD values and total-PD conservation rather than comparing a positional index against an inequality.
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Every review finding on this PR is now addressed on #235, which carries this branch merged onto current Status of the five inline findings:
No Codecov finding needed code — the Codecov checks passed on this revision, and SonarCloud reported a passing quality gate. |
Co-authored-by: coderabbitai[bot] <136622811+coderabbitai[bot]@users.noreply.github.com> Signed-off-by: Jonathan D.A. Jewell <6759885+hyperpolymath@users.noreply.github.com>
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🤖 Completed: Generate docstrings for PR #230 — View commit |
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🤖 Completed: Fix CodeRabbit issues in PR #230 — View commit |
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| Unplaced reads (carried by no tip — like-weight mass left on no edge, plus | ||
| ASVs the tree does not cover — and held out of the weighted UniFrac | ||
| denominators):{' '} |
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Say that unplaced reads remain in the denominator
Whenever a sample has unplaced reads, this user-facing explanation states the opposite of the implemented normalization: src/server/routes/clade_cumulus.jl builds sample_totals from all table reads and passes those totals as the weighted UniFrac denominators, while only the tip abundance is reduced. Researchers are therefore told that the missing mass is excluded from the denominator when it is actually included there but carried by no tip; update the copy to describe that distinction accurately.
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`main` landed its own answer to most of #230 while this branch was open: #236 adopted the asymmetric placement tolerance (shortfall tolerated to 0.05, excess rejected at 1e-9) and #237 added the unplaced-reads noise floor. So this merge gives up re-litigating the tolerance and keeps `main`'s model verbatim: `PLACEMENT_WEIGHT_SUM_TOL = 0.05`, `PLACEMENT_WEIGHT_OVER_TOL = 1e-9`, the asymmetric parse branch, and the partition expectations that follow from it (0.96 is a truncated candidate list and is `:valid`; 1.04 is overweight evidence and stays `:undecided`). What is kept from this branch is what `main` does not have: * the library-size denominators (`_sample_total`, `totals` / `total_a` / `total_b`) that stop weighted UniFrac renormalising unplaced mass back onto the tips that were placed -- the Codex P1 finding; * the route's whole-table `unplaced_reads`, which counts an ASV the tree does not cover at all -- the Codex P2 finding -- instead of summing per-row placement shortfalls. It subsumes #237's noise floor: subtracting the placed mass from the library size reports a 0.99 placement's 1% exactly, with no threshold to tune; * the emptied-clade fix (`own_count`, `own_residual`) and its regression test; * the exact method+path route assertions; * the corrected unplaced-reads wording in the panel and the API types. The single-candidate reason stays `single_candidate_below_min_lwr (<lwr>)`: under `main`'s asymmetric rule a lone candidate's sum is in tolerance by construction, so the old wording named a weight-sum error that had not happened. Co-authored-by: arena-agent <297053741+arena-agent@users.noreply.github.com>
…last child and clarify unplaced-read normalization
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Resolves the merge conflicts on #223 and addresses the review findings raised on it.
mainlanded #222 while #223 was open. The two changes repaired an overlapping set of CladeCumulus defects by different routes, so #223 wentCONFLICTING. This branch is #223 merged into currentmain(8df1041), with each fix kept exactly once, the one semantic disagreement settled, and the Codex P1 finding fixed.Supersedes #223 — the review history lives there; this is the mergeable form of it.
1. Conflicts git could see
read_label_and_quotedNHX capturemain's keyword signature — the call sites already pass keywords — with #223's clearer comment about a tag sitting directly after)PH.Tools→MetaManifold.Toolsin the FastTree testsetPhylogenycannot reach the registry2. The conflict git could not see
#222 widened
PLACEMENT_WEIGHT_SUM_TOLfrom1e-5to0.05so that a lone0.98placement would be accepted. #223 left the tolerance at1e-5and added an explicit single-candidate rule instead. Git merged both halves cleanly and produced a rule neither side intended: the0.05slack survived and now applied only to multi-candidate partitions, which is exactly where it is least wanted — a truncated0.96partition would have passed as complete evidence.Resolved to #223's two-rule model, because it separates the two questions the one tolerance was conflating:
PLACEMENT_WEIGHT_SUM_TOL(1e-5— a floating-point tolerance on an exact identity, not slack for missing mass);PLACEMENT_SINGLE_MIN_LWR(0.5).A lone
0.98still passes; a truncated0.96partition is caught again.3. Codex P1 — preserve unplaced LWR mass in diversity weights
Correct, and it is a defect #223 introduced: before it, a single candidate had to carry essentially all the mass, so a weight of
1.0was right. Admitting a0.5placement madecand.like_weight / twsilently upgrade a coin flip to certainty./clade-cumulusdiversity block now weights each tip by the raw like-weight ratio. For a multi-candidate partition that is the same number to within1e-5; for a single candidate it is the actual share, and the remainder stays off the tree.unplaced_readsfield reports, per sample, the reads the placement file left on no edge. It is surfaced inCladeCumulusPanelunder Faith's PD and typed as optional inapi/types.ts._graft_jplace_queriesis deliberately not changed. The clade tree is a count view of the observed ASV table and conserves every read (root.cumulative_count, an invariant the suite already pins); only the abundance-weighted diversity matrix discounts unplaced mass. The two consumers and why they differ are documented at the constants.4. Carried from #223 unchanged
module DOIopener insrc/core/doi.jl(also fixed by Fix CladeCumulus testset: field names, NHX capture, LWR tolerance, route loading #222 — merged, not duplicated).includeofroutes/cladistics.jlinsrc/server/server.jlremoved.edge_number/like_weight/distal_len/pendant_lenfield names in the jplace graft path and the route's diversity block.scripts/check-julia-syntax.jlrejects a bareendat the top level — the breakage class that let the missingmodule DOIopener through the parse gate and fail at lowering.test/unit/test_routes.jlasserts no method+path pair is registered twice (rewritten to report which route doubled up, and without shadowingBase.pairs).5. Tests added
0.60and at exactlyPLACEMENT_SINGLE_MIN_LWRare valid,0.49is undecided; partitions at0.96and1.04are undecided, and0.60 + 0.60stays undecided so the single rule cannot leak into the many..jplacestudy tree, drives it throughPOST /clade-cumulus, and asserts both the reportedunplaced_reads(ASV_1 placed once at LWR 0.60 → SampleA 40 × 0.4 = 16 unplaced) and that the clade tree still conserves all 110 reads.Validation
Frontend:
tsc --noEmit -p .clean,vite buildclean.Julia is not installable in this environment — the pinned runtime lives on
julialang-s3, which is unreachable, and the GitHub mirror ships source only. Static evidence instead: tree-sitter parses all six touched Julia files with zero ERROR/MISSING nodes, everyQueryCandidate/PhyloNodefield access insrc/andtest/resolves against the current struct definitions, and no route file is now included more than once. CI on this branch is the real check and I will follow it.One standing scanner note:
panic-attackflagsDynamicCodeExecutiononscripts/check-julia-syntax.jlforMeta.parse. That is the script's entire purpose — it parses without evaluating — and the finding predates this branch.