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Fixed bug from #52 causing 'doench_featurization.py' to crash when fed ambiguous nucleotides. - #53

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Fixed bug from #52 causing 'doench_featurization.py' to crash when fed ambiguous nucleotides.#53
mjfos2r wants to merge 4 commits into
USDA-ARS-GBRU:mainfrom
mjfos2r:main

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@mjfos2r

@mjfos2r mjfos2r commented Jun 21, 2025

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Fixed bug from #52 causing 'doench_featurization.py' to crash when fed ambiguous nucleotides. Expanded the fix from v0.4.2 #39.

Also fixed biopython deprecation warning for 'feature.strand' -> 'feature.location.strand'.

Also fixed '.gitignore' since mangling at the bottom was causing it to complain when anything 'guidemaker/*' was added.

Trailing spaces were also automatically removed from all changed files.

Finally, detailed fixes in changelog.

mjfos2r added 4 commits June 20, 2025 20:22
… nucleotides. Also fixed biopython deprecation warning for 'feature.strand' -> 'feature.location.strand'. Also fixed '.gitignore' since mangling at the bottom was causing it to complain when anything 'guidemaker/*' was added. Finally, detailed fixes in changelog. Fixes USDA-ARS-GBRU#52.
… for cases where a gene may not have a viable PAM in the first 500bp, blocking elongation at low efficiency is better than nothing?
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mjfos2r commented Jun 30, 2025

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Not sure why it pulled in the rest of my commits, only merge b9fba51.

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