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docs: add SEED-006 guidance, C-amidation flag, and MDR strain panel to WET_LAB_HANDOFF (#40) - #40
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…ANDOFF Three additions: 1. MDR strain panel table: recommends MRSA USA300, E. coli ST131, KPC-KP ATCC BAA-1705 for Wave 1 alongside ATCC reference strains. Any hit against MDR strains is immediately publishable as clinically significant. 2. C_AMIDATION_RECOMMENDED flag explanation: directs synthesis team to specify 'C-terminal amide (CONH₂)' in the synthesis order form. Flagged for SEED-004/006 variants with neutral C-termini and charge < 3.0. 3. SEED-006 (Mastoparan-X) section: explains the wasp venom calmodulin- binding mechanism, expected Gram-positive bias, hemolysis protocol, synthesis notes (amidation strongly recommended), and interpretation guidance for novel mechanism confirmation.
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Summary
Updates
docs/WET_LAB_HANDOFF.mdwith three actionable wet-lab additions:1. MDR strain panel table
Expands the MIC assay section to explicitly recommend testing against MDR clinical strains alongside ATCC reference standards:
Adds ~3× material cost but dramatically increases publication impact. Any candidate with MIC ≤ 8 μg/mL vs MRSA or KPC-KP qualifies as clinically significant.
2.
C_AMIDATION_RECOMMENDEDflag explanationThe presynth QC report (PR #36) now flags candidates whose C-terminus is neutral and charge pH 7.4 < 3.0. This entry in the flag table directs the synthesis team to request "C-terminal amide (CONH₂)" in the synthesis order form. Action costs nothing at SPPS ordering; adds ~+0.7 net charge and improves serum stability.
3. SEED-006 (Mastoparan-X) special notes section
Adds a dedicated section explaining:
No code changes
Documentation only. All 872 tests pass on
main.Test plan
pytest— 872/872 passed (no code changes)🤖 Generated with Claude Code