diff --git a/docs/CHANGELOG.md b/docs/CHANGELOG.md
index 526b1643..ddcf579e 100644
--- a/docs/CHANGELOG.md
+++ b/docs/CHANGELOG.md
@@ -7,6 +7,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## Unreleased
 
+- Fixed a bug where `rsconnect deploy notebook --static` failed with `Unable to
+  include the file <version> in the bundle: No such file or directory`. The
+  Python version string recorded for the manifest was passed to `nbconvert` as
+  if it were the local interpreter path; the actual interpreter is now used.
 - Fixed a bug where `rsconnect deploy manifest manifest.json` (and other deploy
   commands given a bare filename) failed to detect git metadata. The directory
   passed to git detection was empty in that case, which caused detection to be
diff --git a/rsconnect/main.py b/rsconnect/main.py
index fc4efe9c..2a78e554 100644
--- a/rsconnect/main.py
+++ b/rsconnect/main.py
@@ -1611,10 +1611,11 @@ def deploy_notebook(
 
     ce.validate_server().validate_app_mode(app_mode=app_mode)
     if app_mode == AppModes.STATIC:
+        assert environment.python_interpreter is not None
         ce.make_bundle(
             make_notebook_html_bundle,
             file,
-            environment.python,
+            environment.python_interpreter,
             hide_all_input,
             hide_tagged_input,
         )
diff --git a/tests/test_main.py b/tests/test_main.py
index 3afe0784..b32ead9a 100644
--- a/tests/test_main.py
+++ b/tests/test_main.py
@@ -2,7 +2,9 @@
 import os
 import re
 import shutil
+import sys
 from os.path import join
+from types import SimpleNamespace
 from unittest import TestCase, mock
 
 import click
@@ -13,7 +15,7 @@
 from rsconnect import VERSION
 from rsconnect.api import RSConnectClient, RSConnectServer
 from rsconnect.json_web_token import SECRET_KEY_ENV
-from rsconnect.main import cli, env_management_callback
+from rsconnect.main import cli, env_management_callback, make_notebook_html_bundle
 
 from .utils import (
     apply_common_args,
@@ -303,6 +305,33 @@ def post_application_deploy_callback(request, uri, response_headers):
             if original_server_value:
                 os.environ["CONNECT_SERVER"] = original_server_value
 
+    def test_deploy_notebook_static_passes_interpreter_not_version(self):
+        # Regression test for #721: deploy_notebook must hand make_notebook_html_bundle
+        # the local interpreter *path* (used to run nbconvert), not environment.python,
+        # which is only a version string and made nbconvert fail with "No such file or
+        # directory".
+        fake_env = SimpleNamespace(python="3.12.1", python_interpreter=sys.executable)
+
+        with mock.patch("rsconnect.main.Environment.create_python_environment", return_value=fake_env), mock.patch(
+            "rsconnect.main.RSConnectExecutor"
+        ) as MockExecutor:
+            ce = MockExecutor.return_value
+
+            runner = CliRunner()
+            args = apply_common_args(
+                ["deploy", "notebook", get_dir(join("pip1", "dummy.ipynb"))],
+                server="http://fake_server",
+                key="FAKE_API_KEY",
+            )
+            args += ["--static", "--no-verify"]
+            result = runner.invoke(cli, args)
+
+        assert result.exit_code == 0, result.output
+        # make_bundle(make_notebook_html_bundle, file, <python>, hide_all, hide_tagged)
+        bundle_args = ce.make_bundle.call_args.args
+        assert bundle_args[0] is make_notebook_html_bundle
+        assert bundle_args[2] == sys.executable
+
     @httpretty.activate(verbose=True, allow_net_connect=False)
     def test_deploy_verify_before_activate(self, caplog):
         # Without --draft or --no-verify, the default flow deploys the bundle as a