Hi John,
hope all is well.
in one of our RAD libraries, there about 30% of reads show non-specific digestion. Probably because we left it too long.
But coverage is pretty deep. Thus it could be defendable to keep these tags as well.
For example by allowing users to specify N in the enzyme sequence. eg: --enzyme AATTN (instead of --enzyme AATTC)
Cheers
yannick
Hi John,
hope all is well.
in one of our RAD libraries, there about 30% of reads show non-specific digestion. Probably because we left it too long.
But coverage is pretty deep. Thus it could be defendable to keep these tags as well.
For example by allowing users to specify N in the enzyme sequence. eg:
--enzyme AATTN(instead of--enzyme AATTC)Cheers
yannick