Issue with csv file from flowjow

[Gabrirai]

Hi there,

I have uploaded my cvs files, after extrapolation of the CD45 positiv population with Flowjow. In the antibodies panel I have the included to the kit mastermix (Human Maxpar) other few antibodies. When I look at the data in cyanus, it looks like as one of the new marker I added doesn´t have any signal. I see the signal with the same csv file in Flowjow.
Do you know why this is happening?
I am happy to discuss this topic further.
Best wishes,
Gabriella

[quirinmanz]

Hi Gabriella,

Your problem is tough to judge without looking at the actual data.
A few questions:

• Does it look like the files are read correctly?
• Are there any warnings?
• If everything looks fine, would you be comfortable with sharing some minimal examples with us?

Best,
Quirin